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config.yaml
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blast:
evalue: "1e-10"
maxTargets: "1"
outFmt: "6 qseqid evalue length mismatch salltitles"
deseq2:
maxfraction: "0.7"
fastp:
qualified_quality_phred: "15"
filter_for_plots:
average_samples: "yes"
maximum_pvalue: "0.05"
minimum_foldchange: "2"
minimum_reads: "100"
make_plots:
colour_of_heatmap: "c(\"white\",\"green\",\"green4\",\"violet\",\"purple\")"
height_in_dendrogram: "1.5"
membership_min: "0.2"
method_of_clustering: "pam"
number_of_clusters: "2"
opt_clust_number: "average_silhouette_width"
refs:
annotation: "annotations/Petunia_axillaris_v1.6.2_proteins.mapman_annotation.txt.gz"
genome: "ftp://ftp.psb.ugent.be/pub/plaza/plaza_public_dicots_04/Genomes/cpa.con.gz"
proteins_fasta: "ftp://ftp.solgenomics.net/genomes/Petunia_axillaris/annotation/Petunia_axillaris_v1.6.2_proteins.fasta"
transcriptome_gtf: "ftp://ftp.psb.ugent.be/pub/plaza/plaza_public_dicots_04/GFF/cpa/annotation.all_transcripts.all_features.cpa.gff3.gz"
result_dir: "results/"
trimmomatic:
LeadMinTrimQual: "3"
TrailMinTrimQual: "3"
adapters: "adapters.fasta"
avgMinQual: "15"
minReadLength: "40"
palindromeClipTreshold: "30"
phred: "-phred33"
seedMisMatches: "2"
simpleClipThreshold: "10"
windowSize: "4"
units: "samples.txt"
working_dir: ""