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Hoohm committed Aug 14, 2019
1 parent 498e1c6 commit 9099c59
Showing 1 changed file with 7 additions and 5 deletions.
12 changes: 7 additions & 5 deletions rules/map.smk
Original file line number Diff line number Diff line change
Expand Up @@ -125,7 +125,7 @@ rule DetectBeadSubstitutionErrors:
output:
data=temp('{results_dir}/samples/{sample}/gene_exon_tagged_bead_sub.bam'),
report='{results_dir}/logs/dropseq_tools/{sample}_beadSubstitutionReport.txt',
#stats='{results_dir}/logs/dropseq_tools/{sample}_beadSubstitutionStats.txt',
stats='{results_dir}/logs/dropseq_tools/{sample}_beadSubstitutionStats.txt',
summary='{results_dir}/logs/dropseq_tools/{sample}_beadSubstitutionSummary.txt'
params:
SmartAdapter=config['FILTER']['5-prime-smart-adapter'],
Expand All @@ -135,15 +135,17 @@ rule DetectBeadSubstitutionErrors:
threads: 5
shell:
"""
export _JAVA_OPTIONS=-Djava.io.tmpdir={params.temp_directory} && DetectBeadSubstitutionErrors -m {params.memory}\
export _JAVA_OPTIONS=-Djava.io.tmpdir={params.temp_directory} && DetectBeadSynthesisErrors -m {params.memory}\
I={input}\
O={output.data}\
OUTPUT_REPORT={output.report}\
OUTPUT_SUMMARY={output.summary}\
REPORT={output.report}\
OUTPUT_STATS={output.stats}\
SUMMARY={output.summary}\
PRIMER_SEQUENCE={params.SmartAdapter}\
NUM_THREADS={threads}
"""

rule DetectBeadSynthesisErrors:
rule bead_errors_metrics:
input:
'{results_dir}/samples/{sample}/gene_exon_tagged_bead_sub.bam'
output:
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