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ATAC-Seq Tutorial

This is a tutorial to analyze an ATAC-Seq count matrix as produced by our ATAC-Seq pipeline. The command requires R Statistics and a number of additional R packages. To install R and the dependent packages just execute:

make install

If you already have R installed or want to install the packages manually just skip this step.

Downloading a published ATAC-Seq count matrix

To download the tutorial data and subset to a small number of samples.

./preprocess.sh

This step produces the sample information table blood.samples and the actual count data atac.data.gz, which are required input files for the tutorial.

Building the ATAC-Seq Tutorial Book

You can build the ATAC-Seq book in HTML using

make book

You can then browse the tutorial content using

firefox _book/index.html

The online version of these HTML files you can find here

Generating R scripts

You can convert the Rmarkdown file to a standard Rscript file using

make atac

Credits

This tutorial was created with contributions from Markus Hsi-Yang Fritz.