DEPRECATED Options

After update 1.1 Seqs-Extractor automatically detects the sequence ID and this option is not required anymore

Running MISA and extracting sequences (keep additional information in each the sequence ID)

For users who needs to use extracted sequences of MISA search in Trinity RNA-Seq differential expression pipeline (Grabherr, M. G et al 2011), Seqs-Extractor allows a running-extraction that generates a .FASTA file whose sequences are named exactly as it was in the input file, maintaining additional information as length and path of the sequences in its IDs.

NOTE: This option works only in RNA-Seq data assembled with Trinity (Grabherr, M. G et al 2011).

1. Type the name of the .FASTA file that you want to use to run the MISA search, here is you query sequences.

Example:

  assembled_by_Trinity.fasta

The final screen will indicate the name of the files (MISA results files and the .FASTA file with the sequences extracted). The sequences in .FASTA file generated will be named with the full title of the sequence obtained from the input file. It will be stored in the same directory you are working.