diff --git a/synapse/api/urls.py b/synapse/api/urls.py
index 6e84b1524faa..1ee5236b0fea 100644
--- a/synapse/api/urls.py
+++ b/synapse/api/urls.py
@@ -30,7 +30,8 @@
 STATIC_PREFIX = "/_matrix/static"
 WEB_CLIENT_PREFIX = "/_matrix/client"
 SERVER_KEY_V2_PREFIX = "/_matrix/key/v2"
-MEDIA_PREFIX = "/_matrix/media/r0"
+MEDIA_R0_PREFIX = "/_matrix/media/r0"
+MEDIA_V3_PREFIX = "/_matrix/media/v3"
 LEGACY_MEDIA_PREFIX = "/_matrix/media/v1"
 
 
diff --git a/synapse/app/generic_worker.py b/synapse/app/generic_worker.py
index 51eadf122dba..e512ee150380 100644
--- a/synapse/app/generic_worker.py
+++ b/synapse/app/generic_worker.py
@@ -27,7 +27,8 @@
     CLIENT_API_PREFIX,
     FEDERATION_PREFIX,
     LEGACY_MEDIA_PREFIX,
-    MEDIA_PREFIX,
+    MEDIA_R0_PREFIX,
+    MEDIA_V3_PREFIX,
     SERVER_KEY_V2_PREFIX,
 )
 from synapse.app import _base
@@ -331,7 +332,8 @@ def _listen_http(self, listener_config: ListenerConfig):
 
                         resources.update(
                             {
-                                MEDIA_PREFIX: media_repo,
+                                MEDIA_R0_PREFIX: media_repo,
+                                MEDIA_V3_PREFIX: media_repo,
                                 LEGACY_MEDIA_PREFIX: media_repo,
                                 "/_synapse/admin": admin_resource,
                             }
diff --git a/synapse/app/homeserver.py b/synapse/app/homeserver.py
index 6302dcd91bd3..00ee6f6c9047 100644
--- a/synapse/app/homeserver.py
+++ b/synapse/app/homeserver.py
@@ -29,7 +29,8 @@
 from synapse.api.urls import (
     FEDERATION_PREFIX,
     LEGACY_MEDIA_PREFIX,
-    MEDIA_PREFIX,
+    MEDIA_R0_PREFIX,
+    MEDIA_V3_PREFIX,
     SERVER_KEY_V2_PREFIX,
     STATIC_PREFIX,
     WEB_CLIENT_PREFIX,
@@ -238,7 +239,11 @@ def _configure_named_resource(self, name, compress=False):
             if self.config.server.enable_media_repo:
                 media_repo = self.get_media_repository_resource()
                 resources.update(
-                    {MEDIA_PREFIX: media_repo, LEGACY_MEDIA_PREFIX: media_repo}
+                    {
+                        MEDIA_R0_PREFIX: media_repo,
+                        MEDIA_V3_PREFIX: media_repo,
+                        LEGACY_MEDIA_PREFIX: media_repo,
+                    }
                 )
             elif name == "media":
                 raise ConfigError(