Analysis of Michaelis–Menten enzyme kinetics using the Lineweaver–Burk equation. Plots the Lineweaver–Burk graph using linear regression and estimates Km + Vmax.
Km: 0.000141811172810182
Vmax: 0.2536312215910251
Coefficient of determination: 0.995776951975508
- Python 3
- Pandas
- Numpy
- Matplotlib
- Scikit-learn
Install python dependencies:
# using pip package manager
$ pip install pandas numpy matplotlib sklearn
Save your data as .csv file. Place the substrate concentration in the 'cS' column and reaction rate in the 'v' column (Example: /sample_data/sample_data.csv ):
| cS | v |
|---|---|
| 0.0035 | 50 |
| 0.0065 | 80 |
| ... | ... |
Input your data as an argument (or run without to use sample_data.csv)
# run with your data
$ python main.py `your_data.csv`
# run with sample_data
$ python main.py