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help.php
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<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en">
<head>
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
<title>locBLAST - Local NCBI BLAST+ Search</title>
<link href="style.css" rel="stylesheet" type="text/css" />
</head>
<body>
<div class="output">
<h2 style="text-align: center;"><i style="font-size: medium">loc</i><span style="color:blue; font-size: large">BLAST</span> - <span style="font-size: large;">local NCBI BLAST</span></h2>
<hr class="heffect" />
<div style="padding: 0 15px 0 15px;">
<a href="index.php" title="Go Back to Home"><div style="padding: 0 5px 0 0;" id="back"></div></a>
<h4 style="text-align:center; color:blue" id="top">Tutorial</h4>
<ol>
<li><p>Open the homepage of the <i>loc</i>BLAST server.</p></li>
<li><p>Select the program <i>blastn</i>, <i>blastp</i>, <i>blastx</i>, <i>tblastn</i>, <i>tblastx</i>, <i>deltablast</i>, <i>psiblast</i>, <i>rpsblast</i>, or <i>rpstblastn</i> according to the query sequence in the <b>Program</b> section.</p></li>
<li><p>Select the appropriate database from the list box present in the <b>Database</b> section. The default database is <i>Non-redundant Protein/Nucleotide Sequences</i> (NR/NT).</p></li>
<li><p>Enter the query sequence as FASTA format in the text box (or upload FASTA format sequence file).</p></li>
<li><p>Select the output format for the BLAST result in the <b>Output</b> section.</p></li>
<li><p>Click the <i>Search</i> button or <i>Advanced Search</i> button (if <b>Algorithm Parameters</b> selected) to perform sequence similarity search.</p></li>
</ol>
<h4 style="text-align:center; color:blue" id="top">Tips</h4>
<ul>
<li><p>Move mouse over hyperlinks and icons for tooltip text description.</p></li>
<li><p>Click hyperlinks and icons for a brief description.</p></li>
<li><p>Carefully change <b>Algorithm Parameters</b> (only if needed) according to the query and BLAST program algorithm.</p></li>
</ul>
</div>
</div>
</body>
</html>